Students in the Bioengineering programme use the laboratories of their two advisers. However, a central facility has been created for general use. Currently, the lab is equipped with an Atomic Force Microscope (AFM), a BioAFM, a growth chamber, In Cell Analyzer 6000 high-content imaging system, and a bacterial culture hood in the Bioengineering laboratory. A fume hood and wet-benches are also available. In addition, a Tissue Culture Facility is in the process of being set up. The Bioengineering Office houses a small conference room, office space for Visiting Scientists, the office of the administrative assistant, and workspace for students/researchers.
Park systems NX-10 AFM
Atomic force microscopes (AFMs), or scanning force microscopes, provide a high-resolution 3-D surface profile using a cantilever with a sharp tip to scan the surface to be imaged. AFM measures the deflection of the lever, not the force itself, with most systems using a laser beam deflection system. Park NX-10 AFM system is designed so that the X-Y scanner scans a sample in two-dimensional space, while the Z scanner moves the tip only in the z direction. X-Y scanner has a scan range of 50x50µm and z scanner of 12µm.
Park NX-10 AFM modes
Standard Imaging - True Non-Contact AFM (Air and Liquid), Basic Contact AFM (Air and Liquid), Phase Imaging, Intermittent (tapping) AFM, Force Modulation Microscopy (FMM), Nano indentation, Conductive AFM, I-V Spectroscopy, Force Distance (F-D) Spectroscopy
Contact Cantilevers : CONTSCR, Spring Constant - 0.2N/m, MSNL-10 and MSCT : Spring Constant - 0.01-0.6 N/m, HYDRA Probes with 5um diameter spheres attached to the lever : Spring Constant - 0.01N/m, Non-Contact Cantilevers:ACTA, Spring Constant - 40N/m
Park Systems XE-BIO AFM
XE-Bio is a powerful 3-in-1 nanoscience research tool that uniquely combines industry's only True Non-Contact AFM with Ion Conductance Microscopy (ICM) and inverted optical microscope on the same platform. Designed for non-invasive in-liquid imaging, the combined imaging capability of AFM, ICM, and inverted optical microscopy makes the XE-Bio ideal for imaging biological samples, such as living cells, in dynamic conditions.
This system has an SPM system on the stage of an inverted optical Microscope. The SPM system has an X-Y flat scanner (100µm × 100µm) and a separate SICM or AFM head with a 25µm z scanner. The SICM probe consists of a glass pipette filled with electrolyte with an Ag/AgCl electrode plugged into it.
SICM can be utilized for obtaining contact-free images of the surface topography of biological samples.
GE InCell Analyzer 6000
The InCell Analyzer 6000 is a high-content, laser line scanning confocal microscope equipped with a scientific cMOS camera, specially suited for use in high content assays such as genome-wide screens.
Camera : sCMOS 5.5Mp (2560x2160 pixels)
Imaging modes : Line confocal (aperture adjustable), widefield, transmitted light (brightfield, DIC and phase contrast imaging)
Laser lines : 405, 488, 561 and 642 nm
Objectives : 10X/0.45 NA, 20x/0.45 NA and 60x/0.95 NA
Plate compatibility : Compatible with 6-, 12-, 24-, 48-, 96-, 384-, and 1536-well microplates
Other features : Temperature control, liquid handling, Slide handling
Software : IN Cell Investigator, IN Cell Miner HCM
The volume of the chamber is 2 m3, with work area of 1 m2. Maximum growing height is 1.372 m. The chamber's programmable controller (Percival's IntellusUltra controller) controls temperature, lighting, humidity and carbon-dioxide content. Natural environmental conditions are created by linking multiple programs. Intensity of the light is programmable upto 1100 µmoles/m2/s. Temperature can be programmed to range from 10°C to 44°C± 0.5. The chamber has a spray nozzle humidifier and a heater for dehumidification.
The bacterial cell culture facility at BE comprises a hortizontal laminar flow hood (Shalom Instruments, India), with a UV sterilization system that provides an aseptic environment. In addition, there is a 37°C incubator shaker (Shalom Instruments, India) for growth and incubation of bacterial cultures, a 4°C refrigerator for storage of stock solutions, reagents etc. and a visible range absorbance spectrophotometer (Spectrum, India) for measuring absorbance/ optical density of bacterial cultures to determine growth, proliferation etc.Back to top
In addition, there are several small laboratory instruments that have been procured at the Bioengineering Lab:
- pH meter
- Precision weighing balance
- Spin coater
- Water bath
- Binocular microscope
- Magnetic stirrer