{"id":68,"date":"2020-09-25T18:18:15","date_gmt":"2020-09-25T18:18:15","guid":{"rendered":"https:\/\/be.iisc.ac.in\/~mkjolly\/?page_id=68"},"modified":"2022-10-29T07:02:49","modified_gmt":"2022-10-29T07:02:49","slug":"research","status":"publish","type":"page","link":"https:\/\/be.iisc.ac.in\/~mkjolly\/research\/","title":{"rendered":"Research"},"content":{"rendered":"\n

Our laboratory currently focuses on following research themes:<\/p>\n\n\n\n

Dynamics of Epithelial-Mesenchymal Plasticity<\/strong> <\/h5>\n\n\n\n
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Epithelial-mesenchymal plasticity (EMP) involves reversible and dynamic cell-state transitions among epithelial, mesenchymal and hybrid epithelial\/mesenchymal (E\/M) phenotypes. We focus on identifying the molecular factors stabilizing hybrid E\/M cell-states, and characterizing the association of EMP with other axes of plasticity, such as tumor-initiation potential, immune evasion etc.  <\/p>\n\n\n\n

Selected Publications<\/a><\/p>\n<\/div>\n\n\n\n

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Mechanisms underlying phenotypic heterogeneity in cell populations<\/strong> <\/h5>\n\n\n\n
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Stochastic cell-state transitions driven by various factors such as epigenetic modifications and asymmetric cell division can enable phenotypic heterogeneity in a genetically identical population. Such heterogeneity can enable bet-hedging, thus  facilitating survival of a population under stress and the relapse of population upon stress withdrawal. We aim to identify different mechanisms and underlying principles enabling such phenotypic heterogeneity in diverse biological contexts.<\/p>\n\n\n\n

Selected Publications<\/a> <\/p>\n<\/div>\n\n\n\n

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Spatiotemporal pattern formation and collective behavior in cell populations<\/strong> <\/h5>\n\n\n\n
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Spatiotemporal dynamics of cellular phenotypes are often driven by their crosstalk with their microenvironment, involving cell-cell communication based feedback loops. We develop multi-scale mechanism-based models as predictive \u2018in silico\u202f<\/em>co-culture\u2019 systems to elucidate the collective emergent dynamics in multiple scenarios: tissue-level patterning, collective cell migration, and tumor-stroma crosstalk. <\/p>\n\n\n\n

Selected Publications<\/a><\/p>\n<\/div>\n\n\n\n

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Single-cell analysis of cell-fate decisions in development and cancer<\/strong><\/h5>\n\n\n\n
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Recent single-cell high-throughput data collection has enabled tracking the trajectories of cell-fate decisions and mapping phenotypic heterogeneity in diverse biological contexts. We examine such single-cell transcriptomic data to identify branching points in decision-making, to characterize subpopulations with specific molecular and functional attributes, and to probe the dynamic evolution of different modules of co-expressed gene programs.<\/p>\n\n\n\n

Selected Publications<\/a><\/p>\n<\/div>\n\n\n\n

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Non-genetic mechanisms of adaptive therapy resistance<\/strong> <\/h5>\n\n\n\n
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Besides genetic mutations, non-genetic mechanisms such as phenotypic switching and stochastic cell-to-cell heterogeneity can enable adaptation to various stresses. For instance, drug treatment can induce a phenotypic switch in a fraction of cells to another state (Lamarckian induction). We aim to elucidate how cells adapt reversibly to various stresses, and how we can design strategies to outcompete these adaptation strategies.<\/p>\n\n\n\n

Selected Publications<\/a><\/p>\n<\/div>\n\n\n\n

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Design principles of cell-fate decision-making regulatory networks<\/strong> <\/h5>\n\n\n\n
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Cell-fate decision-making regulatory networks often have unique features in terms of their emergent dynamics, for instance, a limited number of phenotypes despite their complex and large architecture, and balancing robustness against various perturbations with enabling cell-state switching. We are interested in decoding the topological hallmarks of biological networks, and the functional implications of those hallmarks.<\/p>\n\n\n\n

Selected Publications<\/a><\/p>\n<\/div>\n\n\n\n

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Our laboratory currently focuses on following research themes: Dynamics of Epithelial-Mesenchymal Plasticity  Epithelial-mesenchymal plasticity (EMP) involves reversible and dynamic cell-state transitions among epithelial, mesenchymal and hybrid epithelial\/mesenchymal (E\/M) phenotypes. We focus on identifying the molecular factors stabilizing hybrid E\/M cell-states, and characterizing the association of EMP with other axes of plasticity, such as tumor-initiation potential,Continue reading “Research”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-68","page","type-page","status-publish","hentry","entry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/pages\/68","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/comments?post=68"}],"version-history":[{"count":12,"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/pages\/68\/revisions"}],"predecessor-version":[{"id":1473,"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/pages\/68\/revisions\/1473"}],"wp:attachment":[{"href":"https:\/\/be.iisc.ac.in\/~mkjolly\/wp-json\/wp\/v2\/media?parent=68"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}