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Seminar by Dr. Mahipal Ganji on “Decoding the Stabilizing Energetics of DNA and Their Applications in DNA Nanotechnology and Super-resolution Imaging”
October 9 @ 4:00 pm - 5:00 pm
The Department of Bioengineering (BE) cordially invites you to attend a Departmental Seminar.
Speaker: Dr. Mahipal Ganji
Department name: Dept. of Biochemistry, IISc.
Title: Decoding the Stabilizing Energetics of DNA and Their Applications in DNA Nanotechnology and Super-resolution Imaging
About the Speaker: Mahipal studied Physics at Nizam College Hyderabad, India, and Nanobiophysics at TU Dresden, Germany, followed by a PhD from Bionanoscience department at TU Delft, the Netherlands. After short stint as postdoc at the same institute, did a postdoctoral work at the Max-Planck Institute of Biochemistry, Martinsried Germany. His research is interested in understanding how genome is organized in a small compartment. We develop tools to understand factors that alter genome organization using single-molecule imaging and super-resolution microscopy approaches.
In the realm of biology, DNA within our cells constantly undergoes deformations, unwinding and rewinding, yet remarkably, genetic information is efficiently passed down through generations. In my talk, I will delve into our recent endeavors aimed at unraveling the world of stabilizing energetics within DNA. Specifically, we have developed an innovative multiplexed single-molecule assay, harnessing the power of DNA-PAINT super-resolution imaging and DNA nanotechnology. This advanced imaging technology enables us to conduct unbiased kinetic analyses of multiple stacking interactions in a single experiment. Our data reveals that the stability of individual dinucleotide interactions varies dramatically, allowing us to implicate them in diverse applications. During my presentation, I will explore their implications in understanding the mechanical properties of single-stranded DNA. Furthermore, I will discuss how these discoveries are paving the way for the development of efficient imaging probes and their relevance to the field of DNA nanotechnology.