Students in the Bioengineering programme use the laboratories of their two advisers. However, a central facility has been created for general use. Currently, the lab is equipped with an Atomic Force Microscope (AFM), a BioAFM, a growth chamber, In Cell Analyzer 6000 high-content imaging system, and a bacterial culture hood in the Bioengineering laboratory. A fume hood and wet-benches are also available. In addition, a Tissue Culture Facility is in the process of being set up. The Bioengineering Office houses a small conference room, office space for Visiting Scientists, the office of the administrative assistant, and workspace for students/researchers.
Spinning disk confocal microscopes
The system we have is a high-resolution inverted microscope (Eclipse Ti2-E; Nikon) fitted with a spinning disk (CSU-X1; Yokogawa)
Modules: Spinning disk confocal and Total internal reflection fluorescence (TIRF)
Camera: EMCCD camera (iXON Ultra-897; Andor)
Imaging modes: Confocal, TIRF, Widefield, Transmitted light (brightfield and DIC)
Laser lines: 405, 488, 561, 640nm (Toptica)
Objectives: 10X/0.45 NA air, 60X/1.40 NA oil, 100X/1.40 NA oil and 100X/1.49 NA oil (TIRF)
Plate compatibility: 35mm dish, glass slides
Other features: Temperature control, live-call imaging, time-lapse imaging
Software: NIS Elements
For using the facility, please fill this form.
Atomic Force Microscope (AFM)
Park systems NX-10 AFM
Atomic force microscopes (AFMs), or scanning force microscopes, provide a high-resolution 3-D surface profile using a cantilever with a sharp tip to scan the surface to be imaged. AFM measures the deflection of the lever, not the force itself, with most systems using a laser beam deflection system. Park NX-10 AFM system is designed so that the X-Y scanner scans a sample in two-dimensional space, while the Z scanner moves the tip only in the z direction. X-Y scanner has a scan range of 50×50µm and z scanner of 12µm.
Park NX-10 AFM modes
Standard Imaging – True Non-Contact AFM (Air and Liquid), Basic Contact AFM (Air and Liquid), Phase Imaging, Intermittent (tapping) AFM, Force Modulation Microscopy (FMM), Nano indentation, Conductive AFM, I-V Spectroscopy, Force Distance (F-D) Spectroscopy
Contact Cantilevers : CONTSCR, Spring Constant – 0.2N/m, MSNL-10 and MSCT : Spring Constant – 0.01-0.6 N/m, HYDRA Probes with 5um diameter spheres attached to the lever : Spring Constant – 0.01N/m, Non-Contact Cantilevers:ACTA, Spring Constant – 40N/m
IISc User: Rs. 1000 per hour
External Academic Users: Rs. 2500 per hour
Industry Users: Rs. 8000 per hour
All charges are inclusive of tax.
Timings: 9:30am to 5:30pm