Facilities

BD FACS Celesta

The BD FACSLyric system enhances laboratory productivity with its speed, simplicity, and automation. The BD FACSCelesta™ flow cytometer simplifies multicolor flow cytometry, allowing researchers to leverage instrument and reagent innovations. With increasing lasers and detectors, including options like 405 nm, 488 nm, 640 nm and various configurations such as

BV10: 4 Blue (488 nm)/ 6 Violet (405 nm)

BVR12: 4 Blue (488 nm)/ 3 Red (640 nm)/ 5 Violet (405 nm)

BYV12: 2 Blue (488 nm)/ 4 Yellow-Green (561 nm)/ 6 Violet (405 nm)

BUV12: 4 Blue (488 nm)/ 2 UV (355 nm)/ 6 Violet (405 nm)., there’s a wider range of

fluorophores available, expanding cell analysis possibilities.

BD FACS Lyric

The BD FACSLyric™ System comprises the BD FACSLyric™ Flow Cytometer, optional BD FACS™ Universal Loader, and software workstation. The flow cytometer is available in 4, 6, 8, 10, or 12 colors, featuring blue, red, and violet lasers based on configuration. Laser specifications include 488 nm for blue, 640 nm for red, and 405 nm for violet lasers and Configurations such as:

4-color: 2-laser (blue, red) (3-1)

6-color: 2-laser (blue, red) (4-2)

8-color: 3-laser (blue, red, violet) (4-2-2)

10-color: 3-laser (blue, red, violet) (4-3-3)

12-color: 3-laser (blue, red, violet) (4-3-5)

Park systems NX-10 AFM

Atomic force microscopes (AFMs), or scanning force microscopes, provide a high-resolution 3-D surface profile using a cantilever with a sharp tip to scan the surface to be imaged. AFM measures the deflection of the lever, not the force itself, with most systems using a laser beam deflection system. Park NX-10 AFM system is designed so that the X-Y scanner scans a sample in two-dimensional space, while the Z scanner moves the tip only in the z direction. X-Y scanner has a scan range of 50×50µm and z scanner of 12µm.
Park NX-10 AFM modes
Standard Imaging – True Non-Contact AFM (Air and Liquid), Basic Contact AFM (Air and Liquid), Phase Imaging, Intermittent (tapping) AFM, Force Modulation Microscopy (FMM), Nano indentation, Conductive AFM, I-V Spectroscopy, Force Distance (F-D) Spectroscopy
Cantilevers Available
Contact Cantilevers : CONTSCR, Spring Constant – 0.2N/m,  HYDRA Probes with 5um diameter spheres attached to the lever : Spring Constant – 0.045N/m, Non-Contact Cantilevers:ACTA  Spring Constant – 40N/m, NCHR spring constant: 42N/m
Usage Charges
IISc User: Rs. 1000 per hour
External Academic Users: Rs. 2500 per hour
Industry Users: Rs. 8000 per hour
All charges are inclusive of tax.
Timings: 9:30am to 5:30pm

For AFM bookings, contact: Monisha M, monisham@iisc.ac.in

Park systems XE-Bio AFM

Atomic force microscopes (AFMs), or scanning force microscopes, provide a high-resolution 3-D surface profile using a cantilever with a sharp tip to scan the surface to be imaged. AFM measures the deflection of the lever, not the force itself, with most systems using a laser beam deflection system. Park XE-Bio AFM system is designed so that the X-Y scanner scans a sample in two-dimensional space, while the Z scanner moves the tip only in the z direction. X-Y scanner has a scan range of 100×100µm and z scanner of 25µm.
Park XE-Bio AFM modes
Standard Imaging – True Non-Contact AFM (Air and Liquid), Basic Contact AFM (Air and Liquid), Phase Imaging, Intermittent (tapping) AFM, , Nano indentation, Force Distance (F-D) Spectroscopy, Scanning Ion Conductance Microscopy
Cantilevers Available
Contact Cantilevers : CONTSCR, Spring Constant – 0.2N/m, MSNL-10 and MSCT : Spring Constant – 0.01-0.6 N/m, HYDRA Probes with 5um diameter spheres attached to the lever : Spring Constant – 0.01N/m, 0.045N/m Non-Contact Cantilevers:ACTA, Spring Constant – 40N/m
Usage Charges
IISc User: Rs. 1000 per hour
External Academic Users: Rs. 2500 per hour
Industry Users: Rs. 8000 per hour
All charges are inclusive of tax.
Timings: 9:30am to 5:30pm

For AFM bookings, contact: Monisha M, monisham@iisc.ac.in

Leica Confocal SP8 Microscope

Leica Confocal SP8 excels in biomedical research and material science applications, providing precise three-dimensional imaging and thorough examination of subcellular structures and dynamic processes. They feature a range of lasers including 405nm Diode, Multiline Argon (458nm, 488nm, 514nm),561nm and 647nm, along with various magnifications and detectors, including PMTs and HyD Detectors. Capabilities include confocal imaging, fast live cell imaging with a resonant scanner, 24-hour live cell imaging with controlled temperature and CO2 incubation, tile scan/mosaic imaging with navigation, FRET and FRAP imaging, and fluorescence mode with camera support. The available objectives include 10x, 20x, 40x and 63x.

Usage Charges
IISc User: Rs. 1000 per hour
External Academic Users: Rs. 2500 per hour
Industry Users: Rs. 8000 per hour
All charges are inclusive of tax.
Timings: 9:30am to 5:30pm

For bookings, contact: Vinay Kumar V, vinaykumarv@iisc.ac.in

IN Cell Analyzer 6000

Usage Charges
IISc User: Rs. 1000 per hour
External Academic Users: Rs. 2500 per hour
Industry Users: Rs. 8000 per hour
All charges are inclusive of tax.
Timings: 9:30am to 5:30pm

For bookings, contact: Vinay Kumar V, vinaykumarv@iisc.ac.in

Leica CM1950 Cryostat

The Leica CM 1510 is a compact open-top cryostat in a space-saving design for rapid freezing and manual sectioning of tissue specimens. w. The cryo-chamber temperature is selectable between 0 °C and -30 °C. An actively cooled quick-freeze shelf for up to 10 specimen discs ensures rapid freezing of specimens at a maximum temperature of -44 °C (+3 Kelvin) depending on the selected cryo-chamber temperature (-20 °C to -30 °C). The stainless-steel rotary microtome is virtually maintenance-free and provides an 8° X/Y/Z specimen orientation. The section thickness is adjustable in a range of 1 to 50 µm.

Taitec VD-250R Lyophilizer

VD-250R Lyophilizers are designed for versatile applications including drying of samples such as proteins and freeze drying for analytical purposes. They feature cooling temperatures ranging from -45°C (at no load) to -75°C (at no load), offering flexibility for different sample requirements. The trapping volume varies with options of approximately 500 mL/time, 1000 mL/time, and 2000 mL/time. The system is equipped with six different chambers and Freeze-dried Bottles, enabling simultaneous freezing of six different samples at a time.